Miniaturized DNA Biosensor for Decentralized Breast-Cancer Screening

Abstract

The goal of this project has been to develop and characterize an electrochemical microsystem for the rapid point-of-care genetic screening of breast-cancer. We introduced new electrical DNA biosensing routes for genetic screening of breast-cancer. These include novel nanoparticle-based bioassays, label-free schemes based on the intrinsic electroactivity of DNA and coding protocols for multi-target DNA detection. By addressing the major challenge of signal amplification, our research has led to major improvements in the sensitivity of electrical biosensing of DNA segments specific to breast-cancer gene BRCAl. These new advances have been coupled with new schemes for minimizing non-specific adsorption and discriminating against non-complementary sequences. Such coupling of high sensitivity, specificity, and multi-target detection capabilities permits electrical DNA assays to rival the most advanced optical protocols. The new particle-based detection and coding technologies offer great promise for developing fast, simple, and user-friendly DNA sensing devices for point-of-care breast-cancer testing.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2004
Accession Number
ADA426440

Entities

People

  • Joseph Wang

Organizations

  • New Mexico State University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amplification
  • Assays
  • Biosensors
  • Breast Cancer
  • Couplings
  • Detection
  • Dna Biosensors
  • Genetic Testing
  • Glucose Monitors
  • Materials Laboratories
  • Materials Processing
  • Metallic Nanoparticles
  • Nanoparticles
  • Particles
  • Point-Of-Care Diagnostic Testing
  • Target Detection
  • User Friendly

Fields of Study

  • Chemistry

Readers

  • Computer Vision.
  • Molecular and genetic basis of cancer.
  • Nanocomposite Materials Science

Technology Areas

  • Biotechnology