Landscape Phage Probes for Salmonella Typhimurium

Abstract

We selected from a landscape phage library probes that bind preferentially Salmonella typhimurium cells compared with other Enterobacteriaceae. The specificity of the phage probes for Salmonella typhimurium was analyzed by the phage-capture test, the enzyme-linked immunosorbent assay (ELISA) and the precipitation test. Interaction of representative probes with Salmonella typhimurium was characterized by fluorescence-activated cell sorting (FACS), and fluorescent, optical and electron microscopy. The results show that the landscape phage library is a rich source of highly specific and robust probes for Salmonella typhimurium suitable for long-term use in continuous monitoring devices and biosorbents.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2004
Accession Number
ADA426603

Entities

People

  • Ben Fiebor
  • Eric V. Olsen
  • I-hsuan Chen
  • Iryna B. Sorokulova
  • James M. Barbaree

Organizations

  • Air Force Institute of Technology

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Biological Sciences
  • Biomedical And Dental Materials
  • Chemical Analysis
  • Chemical Reactions
  • Chemical Synthesis
  • Chemistry
  • Electron Microscopy
  • Gammaproteobacteria
  • Gram-Negative Bacteria
  • Liquid Chromatography
  • Microbiology
  • Microscopy
  • Polymer Chemistry
  • Polymeric Films
  • Polysaccharides
  • Proteins

Readers

  • Microbial Pathology
  • Molecular Genetics

Technology Areas

  • Microelectronics