Structural Studies on Toxins and Virulence Factors of Yersinia pestis
Abstract
In this first annual report we present our progress on two different areas, protein production and crystallization. We are working on expression and purification of Yop proteins and their complexes. In the first year we learnt reasons why some of the Yop proteins are not crystallizing. We are using a novel approach of making complexes with synthetic peptides or partner proteins to overcome this problem. In the case of LcrV, we are using the inherent property of LcrV forming a coiled coil complex with LcrG to crystallize the complex.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2004
- Accession Number
- ADA426839
Entities
People
- Subramanyam Swaminathan
Organizations
- Brookhaven National Laboratory