Characterization of a Proposed Novel BRCA2 Interaction
Abstract
Breast cancer is one of the most prevalent human cancers, and a leading cause of death among women. BRCA2 is associated with 30-40% of all hereditary cases, predisposing women to early onset breast cancer. The activity of this gene/protein has mainly focused on its involvement in the processes of DNA repair and recombination. We performed a yeast two hybrid to identify new interacting proteins that may indicate its involvement in other cellular processes. Using a human mammary cDNA library we found three independent clones of the gene tristetraprolin (TTP) to interact with BRCA2. This interaction was mapped to an 80 amino acid stretch of the BRCA2 protein using a yeast interaction mating analysis approach. We next transfected HEK-293 cells with epitope tagged constructs (TTP-FL) to demonstrate an in vivo association. Initial results proved successful in demonstrating this interaction. However, for unknown reasons, this interaction ceased to occur. Despite a step-wise procedure to recreate the original in viva results, we were still unable to generate- a positive result.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2004
- Accession Number
- ADA427026
Entities
People
- Christopher J. Huggins
Organizations
- Mount Sinai Hospital, Toronto