A Laboratory Study in Support of the Pilot Demonstration of Biological Soil Slurry Reactor

Abstract

The research conducted in this project demonstrated that TNT can be removed effectively by native soil bacteria via a co-metabolic process. The aerobic bacterial consoflium developed from the TNT-contaminated soil successfully removed TNT in liquid cultures. Various co-substrates (glucose. succinate, acetate. malate, citrate. and molasses) were studied and evaluated. Among these. molasses was identified as the best substrate for TNT removal. Aerobic optimization studies revealed that a pH of 7.0. a temperature of 22-24 deg C. and an ammonium concentration of 0.5 g/L are optimal for degradation of TNT by the bacterial consoflium. The successful operation of an aerobiclanoxic laboratory-scale soil slurry reactor showed that contaminated soil could be treated. Batch treatment resulted in 100% removal of TNT within 21 days. Semicontinuous treatment also resulted in complete degradation of TNT.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1995
Accession Number
ADA427055

Entities

People

  • C. F. Kulpa
  • J. F. Mannig Jr.
  • R. Boopathy

Organizations

  • Argonne National Laboratory

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Ammunition
  • Bacteria
  • Biodegradation
  • Bioremediation
  • Chemical Synthesis
  • Chemistry
  • Chlorides
  • Chromatography
  • Consortiums
  • Ecotoxicology
  • Fungi
  • Liquid Chromatography
  • Metabolism
  • Metabolites
  • Microbiology
  • Microorganisms
  • Organic Chemistry

Fields of Study

  • Environmental science

Readers

  • Groundwater Contamination Remediation.
  • Rocket Propulsion.
  • Toxicology/Environmental Toxicology