A Novel Strategy for Controlling the Metastatic Phenotype: Targeting the SNAG Repression Domain in the SNAIL Zing-Finger Protein

Abstract

Considerable progress has been made in isolating complexes of SNAP-associated polypeptides critical to understanding the determinants of the SNAIL-SNAG domain/SNAP interaction in vitro and in vivo. We have analyzed our unique panel of HEK293 stable cell lines expressing affinity tagged Ajuba and the LIMD1 proteins and examined differences in the cytoplasmic and nuclear complexes. We have developed useful polyclonal antibodies that sensitively detect and discriminate between Ajuba and LIMD1. We are currently mapping the SNAG/SNAP interaction surfaces. Our future goal is to establish dominant negative proteins able to disrupt the SNAG/SNAP interaction and reactive E-Cadherin to control the metastatic phenotype.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2004
Accession Number
ADA427153

Entities

People

  • Frank J. Rauscher Iii

Organizations

  • Wistar Institute

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Antibodies
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Epithelial Cells
  • Fungi
  • Genes
  • Genetics
  • Immune Serums
  • Mass Spectrometry
  • Neoplasms
  • Phenotypes
  • Protein-Protein Interactions
  • Proteins
  • Targeting

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular Genetics
  • Molecular and Cellular Biochemistry