Optical Imaging of Mammaglobin Expression in Breast Cancer
Abstract
Mammaglobin (MMG) is a breast-specific glycoprotein that is over-expressed in nearly 80% of primary and metastatic breast cancers. The exact biological function of this secreted protein is not known. An important step towards understanding the role of MMG in breast carcinogenesis is to monitor its expression in MMG-expressing tumors. This step could be accomplished by labeling antibodies directed to MMG with radioactive or fluorescent antennas. To evaluate the presence of putative MMG receptors, we also labeled the protein and the truncated peptide analogues with radioactive and fluorescent probes, which were injected in tumor-bearing rodents. We successfully prepared and labeled 4 truncated MMG peptide analogues with near infrared and radioactive probes. In vitro MTT assay shows that one of these compounds is not cytotoxic and do not induce cell proliferation. The in vivo imaging studies by fluorescent imaging and positron emission tomography show similar biodistribution of the probes in the liver and kidneys of nude mice. The fluorescently-labeled anti-MMG antibodies are selectively retained in the target tumor tissue and also the major excretion organs, liver and kidneys.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2004
- Accession Number
- ADA427156
Entities
People
- Samuel Achilefu
Organizations
- Washington University in St. Louis