Pain Transmission in Humans: The Role of Novel Sensory Ion Channels

Abstract

The primary accomplishments of the previous funding period were: 1) The 4.0 kb genomic sequence (-4000 to +1) immediately upstream of the transcriptional start site of the ScnlOa gene was fused to the enhanced green fluorescence protein (EGFP) and microinjected into the nuclei of neurons of dorsal root ganglia (DRG). The -4000 to -2500 region was found to be essential for expression of EGFP; 2) The transcription factor c-Jun was found to bind within the -3100 to -3200 region; 3) At least 5 other transcription factors bind within the -3100 to -3200 region; 4) A large number of putative cDNAs encoding the binding domains of putative transcription factors that interact within the -3100 to -3200 region was isolated using the yeast one-hybrid technique; 5) A large collection of cDNAs encoding wild-type and mutant forms of G alpha, G beta, and G gamma subunits were constructed for future analysis into their role in activating the ScnlOa tetrodotoxin-resistant sodium channel.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2003
Accession Number
ADA427161

Entities

People

  • John D. Notti
  • Robert S. Aronstam

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Carrier Proteins
  • Cells
  • Chemical Compounds
  • Chemistry
  • Coding
  • Dna Sequence Analysis
  • Electronic Mail
  • Fungi
  • Gene Expression
  • Genes
  • Genetic Structures
  • Genetics
  • Mutations
  • Proteins
  • Sequence Analysis
  • Transcription Factors

Fields of Study

  • Biology

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