Evaluation of Intracavitary Chemotherapy Delivery for Treatment of Mammary Carcinoma
Abstract
This project will evaluate paclitaxel chemotherapy delivery from a gel polymer system placed into a wound bed following conservative (marginal) surgical removal of human breast cancers grown in nude mice. This delivery method is proposed to control local tumor disease as well as assist in control of systemic metastasis. We have established 5 human breast cancer cell lines within our laboratory. We have elected purchase and implement a unique (luciferase) imaging system which allows in vivo imaging of tumor growth and metastasis (and subsequently decrease animal use) . Tumor cell lines have been transfected with the luciferase gene In vitro testing of cell lines has established paclitaxel sensitivity and shown a synergistic effect of delivering paclitaxel by the poloxamer polymer for the chemotherapy resistant cell line, MCF-7-ADR. We have begun the simultaneous evaluation of local and systemic toxicity, local, regional and systemic distribution and local and systemic efficacy of locally delivered paclitaxel chemotherapy following tumor removal using the MCF-7-ADR cell line in nude mice. Intracavitary administration of taxol in poloxamer has been well tolerated and resulted in complete control of local tumor regrowth and metastasis following marginal tumor removal. This compared to only marginal tumor control using systemically delivered parent paclitaxel.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2004
- Accession Number
- ADA427167
Entities
People
- William S. Dernell
Organizations
- Colorado State University