Multiparametric Evaluation of Marker Expression
Abstract
This project sought to develop flow cytometric methods for monitoring of nuclear hormone receptor expression, DNA content and nuclear volume in human breast tumors. Data was collected and published on estrogen, androgen and vitamin D receptor expression in breast tumors from female and male patients. In a parallel study, we have sought correlation between expression of nuclear hormone receptors and patient characteristics of breast tumors. A manuscript based on expression of markers and other clinical parameters is being revised for submission. Observations have been made on Metachromatic staining of normal vs. breast tumor nuclei with DAPI. This method is being refined for high-resolution flow cytometric characterization of tumors. We have developed methods for simultaneous monitoring of nuclear volume and DNA content of formal in-fixed/paraffin- embedded breast tumors. A paper published showed the superiority of this procedure for obtaining high resolution plots of nuclear volume vs. DNA content for identification of subpopulations in a tumor. This method could be used for more definitive classification of intermediate grade breast tumors. We have submitted two grant applications for use of this technique for refinement of breast tumor stratification. Our data shows that multiparametric analysis of electronic nuclear volume and other marker expression can be used for high resolution analysis of fresh/frozen and archival breast tumors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2004
- Accession Number
- ADA427241
Entities
People
- Awtar Ganju-krishan
Organizations
- University of Miami