Generation of a Gene Expression Cassette to Enable the Rapid Production of DNA Vaccines
Abstract
Recombinant DNA techniques have been used to engineer a DNA vaccine from a gene derived from Burkholderia pseudomallei. This vaccine was further manipulated to generate a gene expression cassette that can be used to readily generate new DNA vaccines, utilising genetic material from any source. The DNA vaccine was transfected into mammalian cells, grown in vitro, in order to assess the ability of the DNA to provide the cell with the necessary information for it to synthesise the protein encoded within the vaccine. Western blot analysis of these cell extracts resulted in the detection of a protein with a molecular mass predicted for the protein encoded by the DNA vaccine. This evidence suggests that transient transfection of a mammalian cell with the engineered plasmid DNA construct, resulting in the translation of the encoded protein has been successful. This in vitro process effectively mimics that which occurs when the DNA plasmids are used to vaccinate higher mammals.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2004
- Accession Number
- ADA427521
Entities
People
- David Proll
- Penelope J. Gauci
Organizations
- Defence Science and Technology Group