Instant Cell Analysis, BioSPICE, Cellular Devices and Exquisite Detection: Towards an Interactive Biology

Abstract

The overall objective of this project was to rapidly find molecular reagents to interfere or bind to every gene product in the Saccharomyces cerevisiae through the development of new screening and data analytical technologies. Two experimental approaches were taken. Ron Davis developed the haploinsufficiency profiling (HIP) technology wherein two libraries of yeast were constructed one containing strains missing one copy the other strains missing both copies of every gene in yeast (that could be knock out). In place of its missing gene, each strain was engineered with a DNA bar code to identify it. Affymetrix chips were used to measure the relative populations of each strain under different chemical perturbations. Roger Brent worked on build protein aptamers to bind gene products. Arkin's role was in the development of analyses for the Haploinsufficiency data.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2004
Accession Number
ADA427703

Entities

People

  • Adam P. Arkin

Organizations

  • University of California, Berkeley

Tags

Communities of Interest

  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Abstracts
  • Bar Codes
  • Cells
  • Databases
  • Detection
  • Environmental Exposure
  • Fungi
  • Gene Expression
  • Information Operations
  • Military Research
  • Molecules
  • Monitoring
  • Perturbations
  • Quality Control
  • Small Molecules
  • Therapy

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Research Science/Academic Research