Downregulation of Breast Cancer Gene Expression by Small Molecule Drugs

Abstract

I had previously reported the identification of a hairpin polyamide that binds sequence specifically to the core promoter of the Her-2 oncogene and interferes with TBP binding. I had also constructed KRAB-fused transcriptional repressor proteins, which binds to the AP-2 binding site of the Her-2 promoter. I now report my findings on the transcriptional repression and proliferation inhibition carried out by the recombinant repressor proteins. The data presented in this report suggests that these recombinant repressors inhibit the expression of proteins involved in cancer cell survival and proliferation. The overall effect of these proteins results in apoptosis of Her- 2 over-expressing cells. In conclusion, these recombinant proteins have the potential to be used as gene therapeutics for breast cancers that show Her-2 over-expression.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2004
Accession Number
ADA427817

Entities

People

  • Y. V. Gopal

Organizations

  • The University of Texas MD Anderson Cancer Center

Tags

DTIC Thesaurus Topics

  • Apoptosis
  • Breast Cancer
  • Cell Physiological Processes
  • Cell Physiology
  • Chemistry
  • Gene Expression
  • Inhibition
  • Molecules
  • Neoplasms
  • Pcr Testing
  • Programmed Cell Death
  • Proteins
  • Recombinant Proteins
  • Small Molecules
  • Survival
  • Therapy

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics