Differential Gene Expression in Explanted Human Retinal Pigment Epithelial Cells 24-Hours Post-Exposure to 532 nm, 3.0 ns Pulsed Laser Light and 1064 nm, 170 ps Pulsed Laser Light 12-Hours Post-Exposure: Results Compendium
Abstract
The use of laser light for military and commercial applications has sharply increased the likelihood of personnel exposure to laser light during operations. The increased potential for human exposure highlights the fact that there is paucity of basic science at the cell and molecular level concerning the effects of laser exposure of human cells. We assessed the sublethal insult to human retinal pigment epithelial cells using a cadaver organ donor explant system for genes differentially expressed 12 and 24 hours post- exposure using gene expression microarray technology (gene chip). The two experiments presented herein are intended to add to the database of laser-tissue interaction at the molecular level using gene expression profiling as the assessment endpoint. This investigative approach continues to illustrate the use of human explants as an experimental model for assessing laser-induced bioeffects as the cell and molecular level. Additionally, we offer 3 conceptual cartoons outlining our vision for the future progress of laser bioeffects research, metabonomic risk assessment modeling and knowledge building from laser bioeffects data.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2004
- Accession Number
- ADA427864
Entities
People
- John W. Obringer
- Martin D. Johnson
Organizations
- United States Air Force Academy