Structural Inheritance in Yeast
Abstract
The purpose of this project is to identify and characterize examples of structural inheritance in yeast that are not prions. We described the first such phenomenon, Leu (exp p), and have made progress in this first year of funding in getting a nuclear genetic "handle" on its mechanistic basis by identifying strains that do not exhibit Leu(exp p). Our most significant advance in this initial year has been the development of the peroxisome as a system to study structural inheritance. We have screened the yeast deletion collection to identify the entire set of genes required for maintenance of the peroxisome. These strains are then to be intercrossed to attempt to identify unlinked non-complementation of deletion alleles. If cytoduction reconstitutes a peroxisome in such doubly heterozygous deletion diploids, structural templating will have been demonstrated. If unlinked non-complementation is not observed for the peroxisome mutants, other structures of yeast will be examined for structural inheritance. The proposal presented initial experiments suggesting that the vacuole exhibits structural inheritance, but further work has not borne this out. In the course of that vacuole work, however, an interesting mating phenomenon was discovered which, although it doesn't bear on the issue of structural inheritance, will result in a publication.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2004
- Accession Number
- ADA427876
Entities
People
- Brian K. Kennedy
- Daniel Lockshon
Organizations
- University of Washington