The Role of Myoepithelial Maspin in Breast Carcinoma Progression, Diagnosis, and Screening
Abstract
In glandular organs, precancerous state precedes invasive carcinoma. In the breast this state is recognized as DCIS and consists of an epithelial cell proliferation confined by myoepithelial cells. Our laboratory has established cell line/xenografts of myoepithelial cells. Our myoepithelial cell lines inhibit invasion and motility of breast carcinoma lines in vitro largely through maspin. The overall hypothesis of this proposal was how does myoepithelial maspin regulate breast (DClS) carcinoma progression and can its detection in fine needle aspirates (FNA) and in ductal fluid abet diagnosis and screening? The first aim addressed the mechanism of maspin's inhibition of breast carcinoma invasion: We have shown that myoepithelial maspin does bind to plasma membranes of carcinoma cells and inhibit a pathway involved in cellular locomotion. Using differential display and microarray analysis we have further characterized the pathway(s) involved. The second aim utilized maspin antibodies on FNA to identify the myoepithelial component and we have further demonstrated with larger number of samples that this approach is successful. The third aim investigated the levels of maspin in nipple aspirates, in ductal lavage fluid and saliva. We have shown that maspin is both a tumor marker as well as a surrogate intermediate end point marker.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2004
- Accession Number
- ADA427884
Entities
People
- Sanford H. Barsky
Organizations
- University of California, Los Angeles