Arginase: A Novel Proliferative Determinant in Prostate Cancer
Abstract
This project is an investigation of the involvement of the enzyme arginase type II (All) in the pathogenesis and growth of prostate cancer. Having recently cloned the All gene in our laboratory, we unexpectedly discovered that it is expressed at high levels in the normal prostate and even higher in neoplastic prostate samples. The purpose of the present research funded by USAMRMC is to examine the expression of All in a wider range of benign and malignant prostate specimens and cultured cells to determine its usefulness as a novel marker of prostatic neoplasia and the extent of its involvement in cancer pathogenesis. We will also explore whether specific chemical and molecular inhibitors of arginase and several related enzymes in the polyamine metabolic pathway might suppress or arrest the growth of prostate cancer cells in vitro or in vivo. This first annual report describes our progress to date in developing the overexpression and inhibitory vector constructs needed for the research, our initial optimization of gene transfer techniques in prostatic cancer cells, and characterization of arginase and related enzymes in prostate cancer lines of various degrees of differentiation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2004
- Accession Number
- ADA427919
Entities
People
- Wayne W. Grody
Organizations
- University of California, Los Angeles