Development of a Rapid and Sensitive Test for the Detection of Prions in Cultured Cells
Abstract
This project endeavors to create cell culture systems for the sensitive and rapid detection of prions. We have proposed to achieve this task by improving steps and components involved (i) in the infection of cells with prions, (ii) in the amplification of prions by cells, and (iii) in the detection of infected cells, To achieve these aims, we are trying to: 1. Identify or create improved cell lines that are more susceptible to prion infection. 2. Improve the delivery of prions to cells by improving their presentation and method of administration, 3. Increase the level of prion/Prp(exp SC) amplification in the infected cells. 4. Design better ways to detect prion infection in cells, either by increasing the formation of PrP(exp SC) or by devising new, non-PrP(exp SC) surrogate' markers.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2004
- Accession Number
- ADA427943
Entities
People
- Albert Taraboulos
Organizations
- Hebrew University of Jerusalem