Quantifying the Effects of Preventive Foods on the Metabolism of a Prostate Carcinogen in Humans and in Prostate Cells Grown in Culture
Abstract
We are investigating the effects of foods associated with reduced prostate cancer risk on a cooked meat carcinogen known to be associated with elevated cancer risk. Cooked muscle meats contain potent mutagens and carcinogens belonging to the heterocyclic amine class of compounds. One of these, PhIP, is a genotoxic carcinogen that has been shown to cause DNA damage in prostate tissue and prostate tumor formation in rats. We have developed a method to quantify urinary metabolites of PhIP in human volunteers that have been fed a meal of cooked chicken. Using this method, we have shown that PhIP metabolism may be affected by diet and lifestyle factors and that broccoli, soy, and tomatoes may influence the relative amounts of PhIP metabolite excretion. At the cellular level we investigated the metabolism of PhIP in human prostate cancer cells and investigated the relationship between DNA damage and gene expression. This research uses state-of-the-art analytical measurement methods to support conclusions about the role of diet and prostate cancer in humans. Although still preliminary, our results indicate that other components of the diet may have an effect on the metabolism of a commonly-occurring food carcinogen.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2004
- Accession Number
- ADA428020
Entities
People
- James S. Felton
Organizations
- Lawrence Livermore National Laboratory