Recombinational Repair Genes and Breast Cancer Risk

Abstract

To seek novel DNA double strand break (DSB) repair genes that may influence breast cancer risk, phenotype-based saeen for chromosome instability mutations in mice, successfully yielding four mutations. Underlying genes for two of these mutations have been identified and their effects on carcinogenesis have been investigated. chaosl (chromosome aberration occurring spontaneously 1) was the first mutation identified from this saeen. This recessive mutation was linked with a missense mutation in Polq encoding DNA polymerase 0, which is potentially involved in DNA inter-strand crosslink repair. We confirmed that chaosl is a mutant allele of Polq using two genetic approaches. Although the chaos1 allele itself does not confer higher cancer susceptibility, interestingly we observed genetic interaction between chaosl and Atm (ataxia telangiectasia mutated), which has a central role in regulating DSB repair by homologous recombination, and presumably influences breast cancer susceptibility.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2004
Accession Number
ADA428433

Entities

People

  • Naoko Shima

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chromosome Aberrations
  • Chromosomes
  • Coding
  • Eukaryotes
  • Genetic Code
  • Genetics
  • Genomic Instability
  • Lymphocytes
  • Mammary Glands
  • Metabolic Diseases
  • Neoplasms
  • Polymerase Chain Reaction
  • Skin Diseases

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Molecular and genetic basis of cancer.

Technology Areas

  • Biotechnology