Scanning the Human Genome for Novel Therapeutic Targets for Breast Cancer

Abstract

The broad goal of this project is to develop genome-wide RNAi approaches in mammals and to apply these to the discovery of new therapeutic targets for cancer. Specifically, we will generate a library of short hairpin RNA expression constructs (shRNA) that ultimately correspond to every gene in the human genome. These will be made available as a public resource and used internally to screen for genes that are essential to the survival of breast cancer cells but which are dispensable for the survival of normal cells. A subset of these might prove suitable as therapeutic targets for breast cancer therapy. During the first year of funding, two things have become clear. First, although they were not in place at the time of submitting this application, we have largely developed the technologies necessary to pursue the above goal. Second, funding in the Innovator award falls far short of that necessary to achieve the goal. Relevant to the last point, we have been able to leverage the Innovator award with several other funding sources to crate a program, which is capable of meeting the proposed goal.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2004
Accession Number
ADA428528

Entities

People

  • Gregory Hannon

Organizations

  • Cold Spring Harbor Laboratory

Tags

DTIC Thesaurus Topics

  • Biological Sciences
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Dna Microarrays
  • Gene Expression
  • Genetic Structures
  • Genetics
  • Genome
  • Human Genome
  • Hydroxides
  • Microarray Analysis
  • Neoplasms
  • Polymerase Chain Reaction
  • Proteins

Fields of Study

  • Biology

Readers

  • Acoustical Oceanography.
  • Molecular and genetic basis of cancer.
  • Oncology (Cancer Research).