Translational Regulation of PTEN/MMAC1 Expression in Prostate Cancer

Abstract

In this project, we proposed to use a dicistronic expression system to determine whether the long 5'-UTR sequence of PTEN contains internal ribosome entry site (IRES) which can mediate cap-independent translation. In the first year of the study, we have accomplished the proposed work as planed. We found that the long 5'-UTR sequence of PTEN inhibits cap-dependent translation and that a region in the 5'-UTR sequence of PTEN has an activity to enhance the expression of the second cistron in a dicistronic assay. However, during the second year of studies when the 5'-UTR sequence of PTEN was cloned into the promoterless dicistronic vector and tested for its stimulatory activity for the expression of the second cistron, we found that the 5'-UTR of PTEN has a strong promoter instead of the previously proposed IRES. We have now mapped this promoter and it is likely responsible for Constitutive production of the PTEN mRNAs with shorter 5'-UTRs which would be compatible for cap-dependent translation initiation.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2004
Accession Number
ADA428550

Entities

People

  • Jian-Ting Zhang

Organizations

  • Indiana University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Epithelial Cells
  • Genetic Code
  • Materials
  • Neoplasms
  • Nucleic Acids
  • Organelles
  • Production
  • Prostate
  • Prostate Cancer
  • Regulations
  • Schematic Diagrams
  • Sequences
  • Stem Cells
  • Transcription Factors

Readers

  • Molecular and genetic basis of cancer.