Examination of the Contribution of VEGF to the Metastatic Dissemination of c-Myc Overexpressing Breast Cancer Cells
Abstract
Metastasis to the lung is a common occurrence accounting for approximately 60-70% of metastasis. We have developed a transgenic model of lung metastasis using a MMTV-c-myc/MMTV-VEGF bitransgenic mouse system. This system and fluorescent variants thereof have been used to examine the importance of VEGF to the metastatic behavior of these cells. We have shown that, surprisingly, the MMTV-c-myc model in fact exhibits much more metastatic potential than previously appreciated, but that c-myc/VEGF cells produce a dramatic stromal reaction in the lung of mice which results in considerable morbidity. The use of EGFP expressing mice as hosts for red-fluorescent tumor cells has greatly facilitated our ability to examine-the processes of extravasation and local tumor cell spread.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2004
- Accession Number
- ADA428564
Entities
People
- Michael J. Johnson
Organizations
- Georgetown University