Electron Microscopy Analysis of Albumin Leakage Across Blood-Brain Barrier Following Microwave Exposure
Abstract
This effort was to develop the histological techniques required to process brain tissue from microwave exposed rats to identify the brain regions affected. Immunocytochemistry was utilized to detect endogenous albumin that had passed through the blood-brain barrier into the brain tissue surrounding the affected vessels. The four key objectives were met: 1) Test for ultrastructural preservation with new perfusion apparatus and when using the specialized fixative; 2) Test that the specialized fixative would allow for immunocytochemical staining with recommended, commercially available antibody to rat albumin, and especially that the albumin staining was equivalent when a gold labeled antibody was or was not included in the traditional indirect immunocytochemical procedure; 3) Test if gold-labeled immunocytochemical staining was detectable in the electron microscopic samples; 4) Evaluate ultrastructure of brain blood vessels and blood brain barrier. The proposed novel histological approach to detect extravasated albumin in brain tissue at both the light and electron microscopic levels was successfully developed and implemented in positive control subjects.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2004
- Accession Number
- ADA428614
Entities
People
- Clyde F. Phelix
Organizations
- University of Texas at San Antonio