Evaluation of an Anti-rPA IgG ELISA for Measuring the Antibody Response in Mice

Abstract

A recombinant protective antigen (rPA)-based enzyme-linked immunosorbent assay (ELISA) was developed to measure the serological response of female A/J mice after inoculation with the new rPA-based anthrax vaccine. Several fundamental parameters of the ELISA were evaluated: specificity, precision, accuracy, linearity, and stability. Experimental results suggested that the quantitative anti-rPA IgG ELISA could be used to measure antibody levels in female A/J mice and may be useful as a potency assay to monitor consistency of manufacture of a rPA-based vaccine for planned clinical trials.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2004
Accession Number
ADA428618

Entities

People

  • G. P. Andrews
  • S. F. Little
  • S. L. Norris
  • W. M. Webster

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Autonomy
  • Biomedical

DTIC Thesaurus Topics

  • Accuracy
  • Antibodies
  • Biological Products
  • Body Fluids
  • Cells
  • Clinical Trials
  • Data Analysis
  • Immune Serums
  • Information Science
  • Laboratory Animals
  • Linear Regression Analysis
  • Precision
  • Proteins
  • Regression Analysis
  • Rodents
  • Test And Evaluation
  • Vaccines

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular and Cellular Biochemistry
  • Nanoscale Plasmonic Nanotechnology

Technology Areas

  • Biotechnology