Differential Protein Expression in Explanted Human Retinal Pigment Epithelial Cells 24-Hours Post-Exposure 532 nm, 3.0 ns Pulsed Laser Light
Abstract
The use of laser light for targeting devices and weapons has dramatically increased the likelihood that personnel will be exposed to laser energy during military operations. Expanded medical, research, and industrial laser use may lead to excessive risk of exposure to researchers and technicians and also during commercial applications. Further, the nature and importance of the biophysical mechanisms of photon-tissue interaction at such pulse widths and irradiances are not understood at the fundamental cell and molecular level. A human in vitro model for assessing laser-light damage to tissue at the cell and molecular level is desirable for scientific, political and fiduciary reasons. This research assesses the effects of sublethal pulsed laser-light treatment to a human cadaver donor retinal pigment epithelial (RPE) tissue using Western Array screening service with antibodies for 859 known human proteins involved in cell signaling, apoptosis, cell adhesion, kinase and GTPase activity as well as several other functions. Some of these functions have been shown to be important in biological processes that could lead to important biological sequelae such as loss of function, cancer or cell death. This research was done to gain insight into the state of cells at the level of protein expression after laser exposure. To our knowledge this is the first ever application of Western Array screening to laser-bioeffects research.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2004
- Accession Number
- ADA428875
Entities
People
- John W. Obringer
- Martin D. Johnson