Microscale Solid-Phase Extraction System for Explosives
Abstract
A simple, semi-automated, microcolumn solid-phase extraction (SPE) system is optimized for the extraction, preconcentration and HPLC analysis of seven different explosives and explosive derivatives contaminating seawater, river water and well water samples. The microcolumns were constructed from 1/16 in. O.D. PTFE tubing (1 in. =2.54 cm) packed with 0.5-1.5 mg of SPE material, LiChrolut EN or Porapak R. The extraction system consisted of two syringe pumps and several solenoid valves. Optimal detection limits were realized when the sample water flow-rate was maximally increased within the limits of the pump, 5-10 ml/min (despite exceeding the breakthrough threshold of the SPE microcolumn), and when the eluate volume collected from the column was minimized, <5 mul (despite very low recovery percentages).
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 24, 2003
- Accession Number
- ADA428908
Entities
People
- Greg E. Collins
- Joseph Wang
- Matthew K Smith
Organizations
- Nova Research Company (United States)