The Impact of Tyrosine Kinase Signaling on Breast Cancer Development
Abstract
Malignant transformation and progression of human cancer is frequently associated with overabundance of proteins that are involved in normal cellular processes, such as proliferation, differentiation, migration or apoptosis. Examples of such proteins include members of human epidermal growth factor receptor (hEGFR) and cellular Src (c-Src) tyrosine kinase families, which are frequently cooverexpressed in human neoplasms and especially in breast cancer. Results from recent studies using cultured fibroblasts and human breast cancer cell lines have indicated that c-Src and EGFR synergistically interact to promote tumor formation in nude mice xenografts. To check whether this synergism occurs in the more physiological setting of the mammary gland, I am going to test the interaction of these tyrosine kinases in a transgenic mouse model, where MMTV-c-Src and MMTV EGFR transgenic mice will be generated, cross-bred to form bigenic mice, then examinated for tumor formation in mammary gland tissue. If the synergism hypothesis is correct, bigenic mice should develop large tumors more rapidly than single transgenic mice, thus validating the synergism between c-Src and EGFR as a target for future therapies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2004
- Accession Number
- ADA428942
Entities
People
- Nadzeya V. Marozkina
Organizations
- University of Virginia