Gene Environment Interactions in Women with Breast Cancer and Secondary Lung Cancer
Abstract
Radiotherapy has become a standard treatment for breast cancer, however, few studies examined individual susceptibilities to risks from radiation exposure. Lung cancer following breast cancer has been associated with radiation exposure and this increased lung cancer risk has been shown to be even higher with tobacco exposure. We are identifying p53 mutations in breast and lung tumors using the Affymetrix GeneChip system and examining the methylation status of five genes important in the progression of breast or lung cancer. We are also describing ER alpha and ER beta expression using immunohistochemistry. We have received 245 of the expected 300 tumors from cases and controls and all samples have been processed and DNA extracted. 160 samples have been analyzed for p53 mutations, 230 samples have been bisulfite modified for methylation assays, 200 methylation assays have been completed, and 145 breast tumors have been stained for ER alpha. This project may have significant clinical impact by providing additional information on risk levels to women choosing a breast cancer therapy. Additionally, this research may provide new data on the susceptibilities of women with multiple primary cancers and on hormone related gender differences in cancer risk.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2004
- Accession Number
- ADA428946
Entities
People
- Meredith A. Tennis
- Peter G. Shields
Organizations
- Georgetown University