Design of Functional Protein Materials Self-Assembled on Solid-Phase Surface

Abstract

For the construction of intelligent materials using protein molecules, it is essential to assemble protein molecules stably on a solid-phase surface in a highly oriented manner. We have designed novel proteins consist of hydrophobic peptide sequence as a self-assembled structural unit and molecular recognition peptide as a functional unit. These designed proteins were expressed in E. coli and purified with affinity chromatography. The resulting proteins retained their functions on a solid-phase surface and they were applied to biosensing system and cellular engineering.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2003
Accession Number
ADA429662

Entities

People

  • E. Kobatake
  • M. Aizawa
  • M. Mie
  • Y. Yanagida

Organizations

  • Tokyo Institute of Technology

Tags

Communities of Interest

  • Air Platforms

DTIC Thesaurus Topics

  • Adhesion
  • Adhesives
  • Amino Acids
  • Biosensors
  • Biotechnology
  • Cell Engineering
  • Cell Physiological Processes
  • Cells
  • Culture Techniques
  • Engineering
  • Hydrophilic Properties
  • Hydrophobic Properties
  • Materials
  • Molecules
  • Phase
  • Proteins
  • Solid Phases

Fields of Study

  • Chemistry

Readers

  • Microbial Pathology
  • Molecular Genetics
  • Nanocomposite Materials Science