A Rat Model for Human Ductal Carcinoma in Situ
Abstract
Our objective is to develop a rodent model for human DCIS and LCIS in which lesions of diverse phenotypes can be induced and characterized and their malignant potential studied in a linear manner. We tested the effect of the infusion of EGF, Heregulin, FGF, or IGF1 on the proliferation of mammary glands in ovariectomized rats. Infusion of EGF, Her, FGF, and IGF1 resulted in end bud formation and lobuloalveolar development within 72 hours. Intraductal proliferation resulted only in rats treated with IGF1. We determined that treatment with growth factors alone would support neoplastic transformation in ovariectomized rats. EGF or Her were infused into the mammary glands of ovariectomized rats and treated with the chemical carcinogen N-methyl-N-nitrosourea and ovaries transplanted. 75% of the rats in both groups developed mammary cancers. These results indicate that infusion of growth factors into the mammary induces proliferation of different kinds of structures, including end buds, lobuloalveolar structures and intraductal proliferation's and that the growth factors in ovariectomized rats can support neoplastic mammary transformation. Our future plans are to select lesions induced in the presence of different growth factors, develop transplantation lines and characterize their biological behavior and their genetic changes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2004
- Accession Number
- ADA430335
Entities
People
- Satyabrata Nandi
Organizations
- University of California, Berkeley