Identifying Early Events in BRCA2-Related Breast Cancer Using Isogenic Cell Lines
Abstract
Three paired primary cell lines derived from BRCA- mutation carrying women have been established. These lines are B28T, B28N (BRCA2 mutation), B22T and B22N and B23T and B23N (BRCA1 mutation) where T and N indicates that the cells are originated from tumor and normal tissue respectively. After mincing and separation, the cells were transformed with E6/E7. The cells were growth in Basal Epithelial media (BEM) supplemented with Insulin, Epidermal Growth Factor and glucocorticoids for the first 2 passages and subsequently with RPM' medium supplemented with 15% fetal bovine serum (FBS). A few B28T cells expressed AE1/AE3 (MCF-7 positive control). This results indicates that only 5% of B28T cells express epithelial markers and suggest that the other cells are mesenchymal (Vimentin +) or myoepithelial cells (Actin +). CK 8/18 and CK 5/6 were used to look for basal differentiation. The only preparation of cells that was positive for CK5/6 was B28T. We concentrate our efforts on the B28T cell line to amplify the subpopulation of cytokeratin positive cells. This line, when fully characterized, will be of considerable value to the research community. Now that we have established this line, further characterization will now follow. We did not complete our goals for this award, but will continue to work on this line for the coming years.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2004
- Accession Number
- ADA430347
Entities
People
- William David Foulkes