Development of a Microfluidic Device for the Study of Breast Cancer Cell Migration

Abstract

This project focuses on the development of microfluidic chambers for the study of breast cancer cell chemotaxis. In the first phase of the project, we designed, fabricated, and tested the microfluidic chemotaxis chamber, ensuring its compatibility with breast cancer cell culture and observation using fluorescence and DIC microscopy. We began the next phase of the project, investigating the migration of breast cancer cells in epidermal growth factor (EGF). We showed that the concentration gradients of EGF induce the chemotaxis of breast cancer cells in a manner that is dependent on the shape of the concentration gradient profile. As we continue our work in this project, we will use the microfluidic chemotaxis chamber to study, in depth, the chemotaxis of breast cancer cells in superimposed gradients of multiple growth factors.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2004
Accession Number
ADA430352

Entities

People

  • Noo L. Jeon
  • Wajeeh M. Saadi

Organizations

  • University of California, Irvine

Tags

DTIC Thesaurus Topics

  • Albumins
  • Anti-Bacterial Agents
  • Biomedical Research
  • Blood
  • Breast Cancer
  • Cell Line
  • Cell Movement
  • Cell Physiological Processes
  • Cells
  • Culture Techniques
  • Data Analysis
  • Fluorescence
  • Growth Factors
  • Materials
  • Microscopy
  • Migration
  • Neoplasms

Readers

  • Breast cancer cell signaling and growth regulation.
  • Nanocomposite Materials Science
  • Naval Engineering and Maritime Security