Genome Wide Expression Analysis of Breast Cancer in African Americans
Abstract
This project focused on the preliminary investigation of whole genome changes associated with breast cancer in African American (AA) women. This project was funded as a Concept Award from the Department of Defense in 2000. The original aims were to analyze total RNA from tissue that would be surgically removed from breast cancer tissue and non-cancerous breast tissue of three AA women with a positive family history of breast cancer, and three women without a family history of breast cancer. RNA extraction, experimental design, hybridization experiments, and data analyses were completed in conjunction with the DNA micro array facility at the University of Miami Medical School. Unfortunately, it took a very long time to achieve Human Subjects approval from both the Department of Defense and the Human Subjects committee at the medical school (as documented in previous letters), as well as to get agreement on the patient recruitment criteria and the tissue procurement protocol by the consulting surgeons and pathologists. Finally, in June 2004, the investigators enrolled their first subject into the study, and despite setbacks from numerous hurricanes in the Summer of 2004, they have four independent cases enrolled (two with strong family histories of breast cancer), cancerous and self-matched normal tissue obtained, and RNA samples ready to be hybridized to Whole Genome DNA micro arrays in January 2005.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2004
- Accession Number
- ADA430386
Entities
People
- Lisa B. Baumbach
Organizations
- University of Miami