Restoring the Breast Mound Using a Preadipoctye-Seeded Growth Factor-Loaded Polymer Hydrogel System
Abstract
HE OBJECTIVE OF THIS PROPOSAL IS TO DEVELOP A CLINICALLY TRANSLATABLE STRATEGY TO RESTORE THE BREAST MOUND FOLLOWING MASTECTOMY OR LUMPECTOMY SUCH THAT PATIENT QUALITY OF LIFE AND OUTCOMES ARE MARKEDLY IMPROVED. Despite tremendous advances in surgical techniques and ancillary support devices, severe reconstructive limitations exist. A new rehabilitative strategy has emerged, namely the field of tissue engineering is attempting to overcome reconstructive limitations by developing strategies that permit patients to regrow their own breast mounds using autologous adipose tissue cells,- specifically preadipocytes or adipose tissue precursor cells. This multidisciplinary application employs principles of bioengineering, synthetic polymer chemistry, and preadipocyte cell biology to develop an innovative biodegradable scaffold material capable of presenting bioactive molecules in a spatially and temporally controlled fashion leading to de novo adipose tissue formation. We hypothesize that a novel material consisting of biodegradable poly(ethylene glycol) (PEG) hydrogels modified to - present a critical cell adhesion molecule and degradation sequence can be seeded with preadipocytes to promote in vitro adipogenesis. The specific aims are to (1) develop a photopolymerizable and biodegradable PEG hydrogel derivatized with YIGSR and LGPA peptides and to bioactivity, and (2) demonstrate, PA viability, proliferation, and differentiation within the PEG hydrogels.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2004
- Accession Number
- ADA430440
Entities
People
- Charles W. Patrick Jr.
Organizations
- University of Texas at Austin