Identification of HP1 Target Genes Involved in Progression of Breast Cancer
Abstract
Heterochromatin protein 1 alpha (HP1alpha) mRNA and protein have previously been reported to be decreased in the invasive, highly metastatic breast cancer cell lines, MDA-MB-231 and Hs578T. Additionally, the introduction of HP1alpha protein into MDA-MB-231 led to decreased metastatic potential. We hypothesized that the decrease in HP1alpha might be due to dysregulation of HP1alpha gene transcription. To test this hypothesis, we first isolated a genomic DNA fragment containing the HP1alpha gene promoter. Portions of the promoter sequence were transfected into stage-specific breast carcinoma cell lines. The HP1apha promoter was less active in the highly metastatic breast cancer cell line MDA-MB-231 compared with other cell lines. Immunoblotting of cell lysates showed that MDA-MB-231 contained decreased HP1alpha protein as compared with less invasive lines. Another metastatic cell line with decreased HP1alpha protein levels, Hs578T, did not show a decrease in promoter activity. Transfection of mRNA against HP1alpha successfully reduced-endogenous HP1alpha in two human cell lines.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2004
- Accession Number
- ADA430474
Entities
People
- Naoko Tanese
Organizations
- New York University