Cellular, Molecular Signaling and Genetic Alterations After High-Peak Power Pulsed and Ultrawideband Radio Frequency Radiation Exposure

Abstract

The most significant finding occurred after exposure of human 244B lymphoblastoid cells to a high-peak power pulsed ultrawideband transmitted electric field (UWB TEMF) signal, which had given a wide array of largely negative results. The UWB TEMF exposure (average peak power, 100 kV/m; average pulse width, 780 ps) duration was 90 min (intermittent). The UWB TEMF did not cause an alteration in cell cycle distribution, stabilization of the p53 target genes. There was no loss of mitochondrial membrane potential or release of cytochrome C into the cytosol at 6 hr post-exposure (i.e., no apoptosis). However, at 2 hr a number of gene increase and decrease were detected (12,000 gene microarray system). This suggests that the cells were capable of "sensing" the pulsed UWB TEMF. While the induction of the gene transcription factor NF-kB was observed, no evidence was obtained for downstream activity. The UWB TEMF signal may be necessary, but not sufficient to cause coordinated downstream events. FDTD analysis was performed to determine the doses for exposure cells in suspension or surface attached. An unexpected distribution of energy in the medium is described.

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Document Details

Document Type
Technical Report
Publication Date
Feb 22, 2005
Accession Number
ADA430555

Entities

People

  • Bijaya Nayak
  • Martin L. Meltz
  • Mohan Natarajan

Organizations

  • University of Texas Health Science Center at San Antonio

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Air Force
  • Air Force Research Laboratories
  • Cell Physiological Processes
  • Cells
  • Chromosomes
  • Electric Fields
  • Electromagnetic Fields
  • Electromagnetic Radiation
  • Frequency
  • Intracellular Membranes
  • Medical Personnel
  • Membrane Potentials
  • Peak Power
  • Proteins
  • Radiation
  • Radio Frequency
  • Transcription Factors

Readers

  • Radar Systems Engineering.
  • Superconducting Magnet Technology
  • Toxicology/Environmental Toxicology

Technology Areas

  • Biotechnology