The Role of Human Spectrin SH3 Domain Binding Protein 1 (HSSH3BPl) in Prostatic Adenocarcinoma
Abstract
Prostate cancer is one of the leading causes of cancer-related deaths in the United States and a leading diagnosed cancer in American men. Newly diagnosed cases of prostate cancer approach rapidly the number of 200, 000 cases per year. Genetic alterations of tumor suppressor genes are one of the most common causes of prostate cancer tumorigenesis. Our group identified Hssh3bpl as a candidate prostate tumor suppressor gene. In this research we are testing the tumor suppressor function of the candidate gene in pro static adenocarcinoma using in vitro and in vivo assays. Using developed cell lines expressing Hssh3bpl we established a candidate region of Hssh3bpl responsible for growth regulation. Within this region we mapped a tyrosine phosphorylation site by c-Abl tyrosine kinase in vitro. This year we established that the same site is phosphorylated in transfected cells and that the phosphorylation of Hssh3bpl is tightly regulated. Further work is under way to develop a detail understanding what is the mechanism of growth regulation by Hssh3bp1 and c-Abl kinase. It is hoped that this work will help us to understand prostate cancer tumorigenesis and ultimately lead to better diagnosis and therapy strategies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2004
- Accession Number
- ADA430579
Entities
People
- Jiliu Xu
- Jill Macoska
- Leszek Kotula
- Magdalena Martinka
- Piotr Kozlowski
Organizations
- Research Foundation For Mental Hygiene