Reconstruction of Mammary Gland Structure Using Three-Dimensional Computer-Based Microscopy
Abstract
During the administrative funding period of this grant we have developed a system that permits three-dimensional reconstruction of entire the entire murine ductal epithelium from physical tissue sections. The system reduces the interaction required for low- resolution imaging of H&E stained sections, registration of images of consecutive sections and annotation of tissue structures -i.e. morphologically normal ducts and intraductal tumors-in the images. In addition, we have developed fully automatic tools for image registration and annotation that are now being integrated in our system and used in the reconstruction of the latest tissue specimens. Complementing morphological H&E based reconstruction, our system can be used for morphologically driven acquisition of high- resolution images from immunostained intermediate sections, both using fluorescence and brightfield microscopy. To show the use of our system, we have used it to compare the whole-gland distribution of hormone receptors (ER and PR) in the development of the mouse mammary gland. For that, we have imaged and quantified the whole-gland cell-by-cell ER and PR status of the mammary gland as it evolves through puberty to maturity and into menopause.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2004
- Accession Number
- ADA430580
Entities
People
- Carlos O. De Solorzano
Organizations
- University of California, Berkeley