Role of CYP1B1 in PAH-DNA Adduct Formation and Breast Cancer Risk
Abstract
The cytochrome P450 enzyme CYP1B1 is a major enzyme involved in metabolizing polycyclic aromatic hydrocarbons (PAHs) to reactive intermediates. Given that certain exposure to PAHs may be a breast cancer risk factor, we have set out to examine CYP1B1 gene expression in breast tissue. After considerable delays in obtaining approval from the Brazilian National Ethics Committee for our protocol for human subject use the project is now operational. A laboratory has been set up in Porto Alegre. The questionnaire and protocols for specimen collection and processing have been finalized. The newly hired Research Coordinators and interviewers have been trained and recruitment of participants has begun. The method to measure CYP1B1 expression has been improved by developing a quantitative RT-PCR assay using a quantitation standard. This quantitation standard was produced by cloning the CYP1B1 PCR fragment into a vector and a dilution series of this standard will be used in each experiment to quantify the absolute amount of CYP1B1 transcripts. Because of the delayed start of the project, we have applied for and obtained a no cost extension.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2004
- Accession Number
- ADA430606
Entities
People
- Regine Goth-goldstein
Organizations
- University of California, Berkeley