Sensitivity of Spores of Eight Bacillus Cereus Strains to Pressure-Induced Germination by Moderate Hydrostatic Pressure, Time and Temperature
Abstract
The spores of eight Bacillus cereus strains were pressurized at 138 to 483 MPa for 5 to 20 min at 25 to 70 C in order to determine the sensitive and the resistant strains to pressure-induced germination. The most sensitive strain was B. cereus QMB 476, while the most resistant strain was B. cereus ATCC 11778. Pressurization at 25 C induced spores to germinate ranged from 2.6 to 5.0 log cycles. An addition of 1.3 to 1.9 log cycle increased in pressure-induced germination of B. cereus, when spores were pressurized at 50 C. Pressurization at 483 MPa for 20 min at 70 C, spores of B. cereus QMB 476 were inactivate from 11.9 to 4.0 log cycles (7.9 log reductions) and spores of B. cereus ATCC 11778 were killed from 9.2 to 3.1 log cycles, 6.1 log reductions. The survivors were destroyed, when spores were pressurized in the presence of bacteriocin-based preservatives containing 100 microng/ml of lysozyme and 500 microng/ml of Na-EDTA.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2005
- Accession Number
- ADA430742
Entities
People
- Anthony Sikes
- Bibek Ray
- C. P. Dunne
- Norasak Kalchayanand
Organizations
- University of Wyoming