Proteomic Analyses of Nipple Fluids for Early Detection of Breast Cancer
Abstract
The purpose of our work is to combine the well-established method of breast nipple fluid collection with the new proteomics methodology for analyses of complex protein mixtures, in order to seek a better test for early breast cancer. The scope of our work is far-reaching, as our results could have a significant impact on the ability to detect occult breast cancers at earlier stages than is possible with current cancer detection methods. Our progress to date has been: 1. to work out a collection method that is efficient and obtains a high-yield of nipple aspirate fluid; 2. to determine that resuspending the aspirate in a small amount of PBS allows for full recovery of the sample; and 3. to develop a systematic protocol for collection that is easily adaptable to other sites. In summary, we have developed a workable protocol for nipple aspirate collection that produces consistent quantitative protein analysis. We are looking forward to proceeding with sample collection for aims one and two once we have received lRB approval from the Department of Defense.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2004
- Accession Number
- ADA431308
Entities
People
- Alexander Miron
- Anne-renee Hartman
- Frederick P. Li
Organizations
- Dana–Farber Cancer Institute