Y Chromosome Aberrations in Prostate Cancer
Abstract
We hypothesize that chromosomal loss of the Y chromosome in prostate cancer is a specific tumorigenic event. Transfer of the Y chromosome by microcell mediated chromosome transfer into PC-3 cells resulted in suppression of tumor growth. However, these PC-3 hybrids containing the Y chromosome did grow in soft agar indicating a lack of correlation between in vitro and in vivo tumor growth. Prom hybrid cells with fragments of the Y chromosome we concluded suppression of tumorigenesis correlates with either the short arm of the Y or two small regions on the q arm. The data from the functional assay was correlated with data from deletions in patient material. Using a complete genomic microarray specific for the Y chromosome that we constructed from BAC clones, we performed array CGH (Comparative genomic hybridization) of prostate tumor samples. These studies indicate there is a small region near on the short arm of the Y chromosome near the centromere that is deleted in 50% of tumor cells. The region of functional suppression and the region of deletion coincide indicating that Y chromosome loss is a significant step in prostate cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2004
- Accession Number
- ADA431579
Entities
People
- Susan L. Naylor
Organizations
- University of Texas at San Antonio