The Role of Protein Elongation Factor EEF1A2 in Breast Cancer
Abstract
The overall goal of the project is to explore the idea that protein elongation factor EEF1A2 has an important causal role in breast tumor development and that EEF1A2 gene amplification and protein overexpression can be used as a breast cancer prognostic factor. In addition, we proposed to test the idea that EEF1A2 expression modulates sensitivity to cisplatin and taxol and that EEF1A2-inactivation could be used as a treatment for breast cancer. In the one-year period since funding has been received, we have made progress in the following areas: 1) We have determined that approximately 25% of primary human breast tumor highly express EEF1A2; 2) We identified two human breast cancer cell lines that highly express EEF1A2 (MCF7 and BT474) and two that do not (MCF10A and BT549); 3) We, created MCF10A and BT549 variants that highly express EEF1A2 and determined that EEF1A2 enhances the in vitro growth of theses variants compared to control; 4) We have created an EEF1A2 transgenic mouse; 5) We have created antisense oligonucleotides (AS) and siRNA that specifically inactivate EEF1A2; 6) We determined that the AS and siRNA inhibit the growth of MCF7 in vitro.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2004
- Accession Number
- ADA431692
Entities
People
- Jonathan M. Lee