Prevention of Breast Cancer in IGFBP
Abstract
In this proposal, we hypothesize that inhibition of IGF action by IGFBP-1 will prevent breast cancer in a SV40 Tag transgenic model of breast cancer. We will test this hypothesis with two specific aims: 1) to inhibit IGF action at the mammary epithelial cell by creating transgenic mice that express IGFBP-l under the control of the whey acidic protein (WAP) promoter and 2) to test the ability of IGFBP-l to suppress tumorigenesis by mating these animals with C3/Tag transgenic mice. To date, we have generated two founder lines containing the IGFBP-l transgene and several Fl and F2 animals were analyzed. Unfortunately, while the transgene was clearly integrated into these animals, we were unable to detect expression of IGFBP-l protein. We also generated animals with an insulator sequences. Despite defining 7 additional founder lines, we were unable to demonstrate expression of IGFBP-l mRNA or protein.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2004
- Accession Number
- ADA431788
Entities
People
- Douglas A. Yee
Organizations
- University of Minnesota