Large Scale Genomic Monitoring or Profiling Using a DNA-Based Memory and Microarrays
Abstract
We describe a new method consisting of enzymatic manipulation of genomic DNA or mRNA with DNA microarrays that is capable of monitoring or profiling any organisms presented in a biological sample without priori knowledge of genomic sequences. The method we have developed seeks to store all genomic DNA information in the bacterial communities found in a patch of soil, water or air sample. The goal is to use genomic information at the population or community scale to monitor and detect the existence of new biota (such as pathogens) in the environment. The scope of organisms with their genomic DNA sequenced is fairly small. Thus, much information at the genomic level is not available with conventional techniques. In addition, many organisms are not amenable to laboratory analysis. However, bio-agents used by terrorist group will be a major threat to our national security. The DNA-based memory potentially provides a way to access information from all organisms in a community (airports, train terminals, etc.) to assess impact by human and non-human biomaterials, does not require explicit sequence knowledge, and is quick, flexible, and inexpensive to implement. Thus, it could provide a holistic view of the genomic status of the whole environment.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2004
- Accession Number
- ADA431849
Entities
People
- Junghuei Chen
- Russell Deaton
- Yuzhen Wang
Organizations
- University of Delaware