Examining the Effects of Exercise Training on Tumor Response to Anthracycline-Based Chemotherapy
Abstract
The purpose of this study is to examine the effects of exercise training on tumor response to anthracycline-based chemotherapy. Eighty athymic Fischer 344 mice will be purchased at 21 days and at 26 days of age, MDA-MB-231 carcinoma cells (3x10EXP 6 cells prepared from donor tumors) will be subcutaneously implanted into the right flank of all animals. At 40 days of age, animals will be randomly assigned to one of four groups: (i) exercise alone (n=20), (ii) doxorubicin alone (n=20), (iii) exercise plus doxorubicin (n=20), and (iv) control (n=20). Animals assigned to doxorubicin alone and exercise plus doxorubicin will receive intravenous injections of doxorubicin at 5mg/kg every 7 days. The exercise group will be progressively trained to run at 22m/min at 0% grade for 45 minutes 5 days/week for 8 weeks. Forty eight hours after the final exercise session, all experimental animals will be sacrificed via carbon dioxide anesthetization and cervical dislocation. The primary tumor will be surgically removed, weighed and histologically processed. The primary outcome will be tumor volume measured in two dimensions. Secondary outcomes will be tumor growth delay calculated as the number of days for each individual animal tumor to reach 1000 cubic mm compared with the control group.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2004
- Accession Number
- ADA432983
Entities
People
- Lee W. Jones
Organizations
- University of Alberta