Performance of a Handheld PCR Instrument in the Detection of Bacillus anthracis, Francisella tularensis, and Yersinia pestis: Sensitivity, Specificity, and Effect of Interferents on Assay Results
Abstract
Smiths Detection-Edgewood, Inc. (SDE) developed the Bio-Seeq to provide a portable platform for use by first responders to detect biological threats in civilian areas. The Bio-Seeq is an updated, redesigned version of a small, portable PCR instrument previously known as the HANAA, Handheld Advanced Nucleic Acid Analyzer. We have tested the performance of real-time fluorogenic PCR assay reagents for the detection of three biological threat agents, Bacillus anthracis (BA), Francisella tularensis (FT), and Yersinia pestis (YP). All three assays are sensitive, and specific for the agents they detect. Common household substances (cornstarch, coffee creamer, baking powder, and wheat flour) interfered with the sensitivity of the assay to varying degrees that were consistent across the three assays.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2004
- Accession Number
- ADA433006
Entities
People
- J. R. Bucher
- K. P. O'connell
- P. E. Anderson
Organizations
- Edgewood Chemical Biological Center