Raman and Surface Enhanced Raman of Biological Material
Abstract
In light of recent world events, an emphasis has been placed firmly on the development of rapid biological threat detection techniques for the military as well as for homeland security. Vibrational spectroscopic methods such as Raman and surface enhanced Raman scattering (SERS) provide rapid detailed fingerprint information about the molecular composition of biomaterial in a non-destructive manner. The technology and methodology to acquire and interpret biological Raman spectra has seen tremendous advances over the past several decades. The problem of tissue fluorescence, which overwhelms the Raman signal of most biological samples upon excitation in the visible region, has been largely overcome by instrumentation operating in the near-IR region of the spectrum. In addition, treatment of biomaterials with silver nanoparticles not only enhances the normal Raman signal by several orders of magnitude but also further reduces the fluorescence backgrounds via interaction between the analyte and the metal SERS substrate. This work presents a study into the applicability of qualitative Raman spectroscopy with principal component analysis (PCA) and surface enhanced Raman for quantitative analysis of the biological threat materials associated with the Rapid Agent Aerosol Detection (RAAD) project.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2004
- Accession Number
- ADA433013
Entities
People
- Jason A Guicheteau
- Kristina Gonser
- Steven D. Christesen
Organizations
- Edgewood Chemical Biological Center