In Vivo Imaging of MDR1A Gene Expression
Abstract
Our experience studying the MDRl gene prompted us to initiate work on a novel animal model to study MDRl/mdrl gene expression under a variety of normal and breast cancer-related physiological conditions. With the advent of new bioimaging technology and the advancement of efficient gene targeting strategies, we found an opportunity to apply these state-of-the-art molecular tools to our problem. The work performed with the support of this grant has enabled us to; 1) engineere a targeting vector to allow insertion of a reporter (luciferase or HSV-tk) into the genomic locus of the mouse mdrla gene; 2) create mouse embryonic stem cells in which a gene replacement/knock-in strategy was used to insert luciferase into the mouse mdrl a genomic locus; 3) demonstrate that luciferase expression in these cells requires Cre recombinase to bring luciferase in-frame with the translational start site of the mdrla gene product; 4) show that the recombined configuration of mdrl/LUC, in its cDNA form, encodes a functional protein with luciferase activity, and 5) create both founder and Cre-recombinase expressing mouse strains for use in vivo imaging experiments. Work performed to date has proved the feasibility of this approach. However, further refinements to the model are required.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2004
- Accession Number
- ADA433034
Entities
People
- Timothy W. Synold